UV-Vis (Ultraviolet-Visible) spectrophotometers are frequently used to measure the concentration of different types of chemical compounds that absorb light in the UV and visible areas of the electromagnetic spectrum. The variety of chemical substances that UV-Vis can detect includes: Organic compounds UV-Vis spectrophotometers are commonly used to determine the concentration of organic compounds with conjugated double bonds or aromatic rings. Examples include chromophores such as phenols, nitro compounds, and polycyclic aromatic hydrocarbons. Inorganic compounds Some inorganic compounds absorb light in… Read More »Measurable Chemicals by UV-Vis Spectrophotometer
The following steps must be performed in order to use a spectrophotometer to measure a chemical compound’s concentration: Sample Preparation Dissolve the chemical compound into a solvent solution. The solution must be clean and free of any contaminants that might interfere with the measurement. Select The Appropriate Wavelength Identify the wavelength of light that the substance absorbs, also known as its maximum absorbance. You can find this information in a reference book or by using a UV-Vis spectral database. Choose… Read More »Measuring Chemicals Using Spectrophotometer
Spectrophotometers are extensively applied in various disciplines, including chemistry, physics, biology, medicine, environmental science, and many more. They quantify the intensity of light absorbed or transmitted by a sample at various wavelengths. Primarily, spectrophotometers are employed to quantify the chemical concentrations in a sample or to identify unidentified substances. Check our previous post about the spectrophotometer or also known as UV-Vis spectroscopy. These are some typical uses and applications of the spectrophotometer: Quantitative analysis of chemical substances Spectrophotometers can be… Read More »UV-Vis Spectrophotometer Uses and Applications
Detecting a chemical compound without a spectrophotometer never being easy. That why every chemistry lab is equipped with this analytical device. Spectrophotometer works by separating the spectral of light and measure its component intensity. Since some chemicals absorb light thus the intensity of the adsorption can be measured by using a spectrophotometer and its concentration could be quantified. Light Light is some sort of electromagnetic radiation emitted from its sources. It could be light from the candle, lamp, sunlight reflected… Read More »What is a Spectrophotometer?